Genetic Screening of Halothane Gene on Selected Philippine Native Pig Herds

The establishment of nucleus herds (NHs) Native Pigs (NPs) at various R&D stations in the Philippines is currently being undertaken for food security and genetic conservation advocacy. Marker-assisted selection (MAS) utilized to identify individuals carrying favorable alleles genes associated with production traits screen out defects (GD) breeding purposes. Porcine Stress Syndrome (PSS) caused by a mutation Halothane (HAL) gene GD frequently found commercial breeds that when expressed, causes pale, soft, exudative (PSE) meat. PSE inferior quality meat undesirable market causing economic losses swine industry. Thus, this study was conducted HAL through mutagenically separated-polymerase chain reaction (MSPCR) selected NP assessed its repeatability local breeds. Results showed 577 screened individuals, 543 (94.11%) were normal (NN), 0 (0%) homozygous mutant (nn) 34 (5.89%) heterozygous carriers (Nn). Therefore, optimized PSS screening protocol using MSPCR also applicable As such, availability tests could be useful improving Philippine NPs inhibiting or eliminating incidence within herd.